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. 2015 Jan 26:4:e04105.
doi: 10.7554/eLife.04105.

Registered report: senescence surveillance of pre-malignant hepatocytes limits liver cancer development

Samrrah Raouf  1 , Claire Weston  2 , Nora Yucel  3 ; Reproducibility Project: Cancer Biology
Collaborators, Affiliations

Registered report: senescence surveillance of pre-malignant hepatocytes limits liver cancer development

Samrrah Raouf et al. Elife. .

Abstract

The Kang et al. (2011), published in Nature in 2011. The experiments that will be replicated are those reported in Figures 3B, 3C, 3E, and 4A. In these experiments, Kang et al. (2011) demonstrate the phenomenon of oncogene-induced cellular senescence and immune-mediated clearance of senescent cells after intrahepatic injection of NRAS (Figures 2I, 3B, 3C, and 3E). Additionally, Kang et al. (2011) show the specific necessity of CD4+ T cells for immunoclearance of senescent cells (Figure 4A). The Reproducibility Project: Cancer Biology is a collaboration between the eLife.

Keywords: CD4+ T-cells; Reproducibility Project: Cancer Biology; cell biology; human biology; immune-mediated clearance; medicine; methodology; mouse; oncogene-induced senescence.

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Conflict of interest statement

SR: The UC-Davis Mouse Biology Program is a Science Exchange affiliated lab.

CW: Reveal Biosciences is a Science Exchange affiliated lab.

RP:CB: EI, FT and JL are employed and holds shares in Science Exchange, Inc.

The other authors declare that no competing interests exist.

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References

    1. Acosta JC, Banito A, Wuestefeld T, Georgilis A, Janich P, Morton JP, Athineos D, Kang TW, Lasitschka F, Andrulis M, Pascual G, Morris KJ, Khan S, Jin H, Dharmalingam G, Snijders AP, Carroll T, Capper D, Pritchard C, Inman GJ, Longerich T, Sansom OJ, Benitah SA, Zender L, Gil J. A complex secretory program orchestrated by the inflammasome controls paracrine senescence. Nature Cell Biology. 2013;15:978–990. doi: 10.1038/ncb2784. - DOI - PMC - PubMed
    1. Bell JB, Podetz-Pedersen KM, Aronovich EL, Belur LR, McIvor RS, Hackett PB. Preferential delivery of the Sleeping Beauty transposon system to livers of mice by hydrodynamic injection. Nature Protocols. 2007;2:3153–3165. doi: 10.1038/nprot.2007.471. - DOI - PMC - PubMed
    1. Bennecke M, Kriegl L, Bajbouj M, Retzlaff K, Robine S, Jung A, Arkan MC, Kirchner T, Greten FR. Ink4a/Arf and oncogene-induced senescence prevent tumor progression during alternative colorectal tumorigenesis. Cancer Cell. 2010;18:135–146. doi: 10.1016/j.ccr.2010.06.013. - DOI - PubMed
    1. Braig M, Lee S, Loddenkemper C, Rudolph C, Peters AH, Schlegelberger B, Stein H, Dörken B, Jenuwein T, Schmitt CA. Oncogene-induced senescence as an initial barrier in lymphoma development. Nature. 2005;436:660–665. doi: 10.1038/nature03841. - DOI - PubMed
    1. Brinkhoff B, Ostroumov D, Heemcke J, Woller N, Gurlevik E, Manns MP, Longerich T, Zender L, Harty JT, Kubicka S, Kuhnel F, Wirth TC. Microsphere priming facilitates induction of potent therapeutic T-cell immune responses against autochthonous liver cancers. European Journal of Immunology. 2014;44:1213–1224. doi: 10.1002/eji.201343794. - DOI - PubMed

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Grants and funding

The Reproducibility Project: Cancer Biology is funded by the Laura and John Arnold Foundation, provided to the Center for Open Science in collaboration with Science Exchange. The funder had no role in study design or the decision to submit the work for publication.