Micro-computed tomography and histomorphometric analysis of the effects of platelet-rich fibrin on bone regeneration in the rabbit calvarium

Arch Oral Biol. 2015 Apr;60(4):606-14. doi: 10.1016/j.archoralbio.2014.09.017. Epub 2014 Oct 14.


Objective: The present study aimed to investigate the effectiveness of platelet-rich fibrin (PRF) on bone regeneration when used alone or in combination with hydroxyapatite (HA)/beta-tricalcium phosphate (βTCP).

Design: In this study, 20 New Zealand white rabbits were used and four calvarial defects were prepared in each animal. PRF, Straumann(®) Bone Ceramic (SBC), or PRF+SBC was applied to the defects; one defect was left untreated as a control. Ten rabbits were sacrificed at week 4 (T1) and 10 at week 8 (T2). After micro-computed tomography (micro-CT) scanning, the samples were sent for histological and histomorphometric analysis to evaluate and compare the volume and area of regenerated bone.

Results: Histomorphometric and micro-CT analysis showed that both PRF and SBC significantly increased bone regeneration at T1 and T2 (P<0.01). When PRF was used in combination with HA/βTCP, a further significant increase in new bone formation was observed at T1 and T2 compared with that when PRF or SBC was used alone (P<0.01).

Conclusions: PRF has a positive effect on bone formation when used alone and in combination with HA/βTCP.

Keywords: Beta-tricalcium phosphate; Calvarial defect; Hydroxyapatite; Platelet-rich fibrin; Rabbit.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Blood Platelets / physiology*
  • Bone Regeneration / drug effects*
  • Bone Regeneration / physiology
  • Bone Substitutes / pharmacology*
  • Fibrin / pharmacology*
  • Hydroxyapatites / pharmacology*
  • Rabbits
  • Skull / diagnostic imaging*
  • Skull / surgery*
  • X-Ray Microtomography*


  • Bone Substitutes
  • Hydroxyapatites
  • hydroxyapatite-beta tricalcium phosphate
  • Fibrin