An integrated optofluidic device for single-cell sorting driven by mechanical properties

Lab Chip. 2015 Mar 7;15(5):1262-6. doi: 10.1039/c4lc01496k.

Abstract

We present a novel optofluidic device for real-time sorting on the basis of cell mechanical properties, measured by optical stretching. The whole mechanism, based on optical forces, does not hamper the viability of the tested cells, which can be used for further analysis. The device effectiveness is demonstrated by extracting a sample population enriched with highly metastatic cells from a heterogeneous cell mixture.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Line, Tumor
  • Cell Separation / instrumentation*
  • Cell Shape
  • Cell Size
  • Humans
  • Melanoma / pathology
  • Microfluidic Analytical Techniques*
  • Neoplasm Metastasis
  • Skin Neoplasms / pathology