Pea (Pisum sativum L.) seed albumin extracts show anti-inflammatory effect in the DSS model of mouse colitis

Mol Nutr Food Res. 2015 Apr;59(4):807-19. doi: 10.1002/mnfr.201400630. Epub 2015 Mar 2.

Abstract

Scope: This study investigates the preventive effects of two pea (Pisum sativum) seed albumin extracts, either in the presence (pea seed extract [PSE]) or absence (albumin fraction from PSE [AF-PSE]) of soluble polysaccharides, in the dextran sodium sulfate (DSS) induced colitis in mice.

Methods and results: Male C57BL/6J mice were assigned to five groups: one noncolitic and four colitic. Colitis was induced by incorporating DSS (3.5%) in the drinking water for 4 days, after which DSS was removed. Treated groups received orally PSE (15 g/kg⋅day), or AF-PSE (1.5 g/kg⋅day), or pure soy Bowman-Birk inhibitor (BBI; 50 mg/kg⋅day), starting 2 wk before colitis induction, and maintained for 9 days after. All treated groups showed intestinal anti-inflammatory effect, evidenced by reduced microscopic histological damage in comparison with untreated colitic mice. The treatments ameliorated the colonic mRNA expression of different proinflammatory markers: cytokines, inducible enzymes, metalloproteinases, adhesion molecules, and toll-like receptors, as well as proteins involved in maintaining the epithelial barrier function. Furthermore, the administration of PSE, AF-PSE, or soy BBI restored bacterial counts, partially or totally, to values in healthy mice.

Conclusion: PSE and AF-PSE ameliorated DSS-induced damage to mice, their effects being due, at least partially, to the presence of active BBI.

Keywords: Bowman-Birk inhibitor; Intestinal microbiota; Mouse DSS experimental colitis; Pea protein fractions; Pisum sativum.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Albumins / pharmacology*
  • Animals
  • Anti-Inflammatory Agents / pharmacology*
  • Cecum / drug effects
  • Cecum / microbiology
  • Colitis / chemically induced
  • Colitis / drug therapy*
  • Colon / metabolism
  • Colon / microbiology
  • Cyclooxygenase 2 / genetics
  • Cyclooxygenase 2 / metabolism
  • Dextran Sulfate / adverse effects
  • Disease Models, Animal
  • Gastrointestinal Microbiome
  • Interferon-gamma / genetics
  • Interferon-gamma / metabolism
  • Interleukin-12 / genetics
  • Interleukin-12 / metabolism
  • Interleukin-1beta / genetics
  • Interleukin-1beta / metabolism
  • Interleukin-6 / genetics
  • Interleukin-6 / metabolism
  • Intestinal Mucosa / metabolism
  • Intestines / drug effects
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Pisum sativum / chemistry*
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • Seeds / chemistry*
  • Tumor Necrosis Factor-alpha / genetics
  • Tumor Necrosis Factor-alpha / metabolism

Substances

  • Albumins
  • Anti-Inflammatory Agents
  • Interleukin-1beta
  • Interleukin-6
  • RNA, Messenger
  • Tumor Necrosis Factor-alpha
  • Interleukin-12
  • Interferon-gamma
  • Dextran Sulfate
  • Ptgs2 protein, mouse
  • Cyclooxygenase 2