Ppp2ca knockout in mice spermatogenesis

Reproduction. 2015 Apr;149(4):385-91. doi: 10.1530/REP-14-0231. Epub 2015 Jan 27.

Abstract

Protein phosphatase 2A (PP2A) is a ubiquitous serine/threonine phosphatase involved in meiosis, mitosis, sperm capacitation, and apoptosis. Abberant activity of PP2A has been associated with a number of diseases. The homolog PPP2CA and PPP2CB can each function as the phosphatase catalytic subunit generally referred to as PP2AC. We generated a Ppp2ca conditional knockout (CKO) in C57BL/6J mice. Exon 2 of Ppp2ca was knocked out in a spatial or temporal-specific manner in primordial germ cells at E12.5. This Ppp2ca-null mutation caused infertility in male C57BL/6J mice. These CKO mice provide a powerful tool to study the mechanisms of Ppp2ca in development and disease.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Blotting, Western
  • Cells, Cultured
  • Infertility, Male / etiology*
  • Integrases / metabolism
  • Male
  • Meiosis / physiology*
  • Mice
  • Mice, Inbred C57BL
  • Mice, Knockout
  • Protein Phosphatase 2 / physiology*
  • RNA, Messenger / genetics
  • Real-Time Polymerase Chain Reaction
  • Reverse Transcriptase Polymerase Chain Reaction
  • Spermatogenesis / physiology*

Substances

  • RNA, Messenger
  • Cre recombinase
  • Integrases
  • PPP2CA protein, mouse
  • Protein Phosphatase 2