Abstract
Assembly of foreign prokaryotic ribulose bisphosphate carboxylases (Rubiscos) in Escherichia coli requires both heat-shock proteins groEL and groES. GroEL is related to a chloroplast protein implicated in Rubisco assembly. Bacteria and chloroplasts therefore have a conserved mechanism that uses auxiliary proteins to assist in the assembly of Rubisco.
MeSH terms
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Bacterial Proteins / genetics
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Bacterial Proteins / pharmacology*
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Chaperonin 10
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Chaperonin 60
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Escherichia coli / genetics
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Genes, Bacterial
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Heat-Shock Proteins / genetics
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Heat-Shock Proteins / pharmacology*
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Mutation
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Plasmids
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Ribulose-Bisphosphate Carboxylase / biosynthesis*
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Transformation, Genetic
Substances
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Bacterial Proteins
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Chaperonin 10
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Chaperonin 60
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Heat-Shock Proteins
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Ribulose-Bisphosphate Carboxylase