Abstract
Molecularly imprinted polymers bearing peptide fragment-based binding sites within the protein-imprinted cavities were prepared by copolymerization of the acrylated protein with 6-monoacryloyl-trehalose and 6,6'-diacryloyl-trehalose as a hydrophilic comonomer and a crosslinker respectively, followed by enzymatic decomposition of the grafted protein into the polymer matrix with pepsin, resulting in the creation of peptide fragment-based protein-binding sites.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Binding Sites
-
Cytochromes c / chemistry*
-
Cytochromes c / metabolism
-
Humans
-
Molecular Imprinting / methods*
-
Pepsin A / metabolism*
-
Peptide Fragments / chemistry*
-
Peptide Fragments / metabolism
-
Polymers / chemical synthesis*
-
Polymers / metabolism*
-
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
-
Trehalose / chemistry
Substances
-
Peptide Fragments
-
Polymers
-
Cytochromes c
-
Trehalose
-
Pepsin A