Probing the Molecular and Structural Elements of Ligands Binding to the Active Site Versus an Allosteric Pocket of the Human Farnesyl Pyrophosphate Synthase

Bioorg Med Chem Lett. 2015 Mar 1;25(5):1117-23. doi: 10.1016/j.bmcl.2014.12.089. Epub 2015 Jan 13.

Abstract

In order to explore the interactions of bisphosphonate ligands with the active site and an allosteric pocket of the human farnesyl pyrophosphate synthase (hFPPS), substituted indole and azabenzimidazole bisphosphonates were designed as chameleon ligands. NMR and crystallographic studies revealed that these compounds can occupy both sub-pockets of the active site cavity, as well as the allosteric pocket of hFPPS in the presence of the enzyme's Mg(2+) ion cofactor. These results are consistent with the previously proposed hypothesis that the allosteric pocket of hFPPS, located near the active site, plays a feed-back regulatory role for this enzyme.

Keywords: Allosteric inhibitors; Bisphosphonates; Human FPPS.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Allosteric Site
  • Catalytic Domain
  • Diphosphonates / chemistry
  • Diphosphonates / metabolism*
  • Geranyltranstransferase / chemistry*
  • Geranyltranstransferase / metabolism*
  • Humans
  • Ligands
  • Magnesium / metabolism
  • Molecular Docking Simulation
  • Protein Binding

Substances

  • Diphosphonates
  • Ligands
  • Geranyltranstransferase
  • Magnesium