Oral administration of Bifidobacterim bifidum for modulating microflora, acid and bile resistance, and physiological indices in mice

Can J Microbiol. 2015 Feb;61(2):155-63. doi: 10.1139/cjm-2014-0694. Epub 2014 Nov 21.


Bifidobacteria are generally acknowledged as major gut microflora used as probiotics, which promote human health. In this study, the effects of the administration of Bifidobacterim bifidum on modulating gastrointestinal (GI) tract microflora, acid and bile resistance, and physiological indices in BALB/c mice were investigated. Results showed that B. bifidum can significantly improve the ecosystem of the GI tract by increasing the amount of probiotics and reducing the populations of pathogenic bacteria, as measured by plate count and real-time PCR. After exposure to simulated GI tract conditions, the growth of gut microflora in the B. bifidum group was higher than that in the control group when incubated for 12 h in MRS or nutrient broth adjusted to pH 2.0 or 3.0 or in the presence of a concentration of bile salt (0.45% m/v). The blood biochemical index was examined, and the physiological effect of the cell-free extract of gut microflora was evaluated by measuring the activity of various enzymes, including α-glucosidases, esterase, and lactate dehydrogenase. This study suggested that a B. bifidum strain can stabilize blood sugar, lower cholesterol levels in serum, and improve metabolic activity. Moreover, B. bifidum was a promising enhancer of microbial diversity in mouse intestine and played a vital role in human physiological processes, which can benefit the health of a host.

Keywords: B. bifidum; acid and bile resistant; diversité microbienne; gastrointestinal tract; microbial diversity; résistant à l’acide et à la bile; tractus gastro-intestinal.

MeSH terms

  • Administration, Oral*
  • Animals
  • Bifidobacterium*
  • Bile
  • Bile Acids and Salts / chemistry
  • Cell Survival
  • Female
  • Gastrointestinal Microbiome*
  • Gastrointestinal Tract / microbiology
  • Hydrogen-Ion Concentration
  • L-Lactate Dehydrogenase / chemistry
  • Male
  • Mice
  • Mice, Inbred BALB C
  • Probiotics / administration & dosage*
  • RNA, Ribosomal, 16S / genetics
  • Real-Time Polymerase Chain Reaction


  • Bile Acids and Salts
  • RNA, Ribosomal, 16S
  • L-Lactate Dehydrogenase