In vivo assessment of mitochondrial oxygen consumption

Methods Mol Biol. 2015;1264:219-29. doi: 10.1007/978-1-4939-2257-4_20.

Abstract

The protoporphyrin IX-triplet state lifetime technique (PpIX-TSLT) has been proposed by us as a potential clinical noninvasive tool for monitoring mitochondrial function. We have been working on the development of mitochondrial respirometry for monitoring mitochondrial oxygen tension (mitoPO2) and mitochondrial oxygen consumption (mitoVO2) in skin. In this work we describe the principles of the method in experimental animals.

MeSH terms

  • Animals
  • In Vitro Techniques / instrumentation
  • In Vitro Techniques / methods*
  • Luminescent Measurements / instrumentation
  • Luminescent Measurements / methods
  • Mitochondria / metabolism*
  • Oxygen Consumption*
  • Rats