Green tea changes serum and liver metabolomic profiles in mice with high-fat diet-induced obesity

Mol Nutr Food Res. 2015 Apr;59(4):784-94. doi: 10.1002/mnfr.201400470. Epub 2015 Mar 3.


Scope: Green tea (GT) consumption helps to prevent and control obesity by stimulating hepatic lipid metabolism. However, GT-induced changes in serum and liver metabolomes associated with the anti-obesity effects are not clearly understood. The aim of this study was to identify and validate metabolomic profiles in the livers and sera of GT-fed obese mice to elucidate the relationship between GT consumption and obesity prevention.

Methods and results: Serum and liver metabolites were analyzed in mice fed normal diet, high-fat diet (HFD), HFD with GT, and HFD with crude catechins, using LC-quadrupole TOF MS. The addition of 1% GT to HFD reduced adipose tissue and the levels of blood triglycerides, glucose, insulin, and leptin elevated in HFD-fed mice. We proposed an HFD-induced obesity pathway and validated it by investigating the key regulatory enzymes of mitochondrial β-oxidation: carnitine palmitoyltransferase-1 and -2, acyl-coenzyme A dehydrogenase, and acetyl-coenzyme A acyltransferase. The results showed that HFD-induced abnormal mitochondrial β-oxidation was moderated by the consumption of caffeine- and theanine-enriched GT.

Conclusion: Results of LC/MS-based metabolomic analysis of obese mice showed changes associated with abnormal lipid and energy metabolism, which were alleviated by GT intake, indicating the mechanism underlying the anti-obesity effects of GT.

Keywords: Acetyl-CoA acyltransferase 2; Fatty acid β-oxidation; Green tea; Metabolomics; Obesity.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acetyl-CoA C-Acyltransferase / metabolism
  • Acyl-CoA Dehydrogenase / metabolism
  • Adipose Tissue / metabolism
  • Animals
  • Blood Glucose / metabolism
  • Carnitine O-Palmitoyltransferase / metabolism
  • Cholesterol / blood
  • Diet, High-Fat / adverse effects*
  • Dietary Fats / administration & dosage
  • Energy Metabolism
  • Insulin / blood
  • Leptin / blood
  • Lipid Metabolism
  • Liver / metabolism*
  • Male
  • Metabolome*
  • Mice
  • Mice, Inbred C57BL
  • Mice, Obese
  • Mitochondria / drug effects
  • Mitochondria / metabolism
  • Multivariate Analysis
  • Obesity / diet therapy*
  • Obesity / etiology
  • Tea / chemistry*
  • Triglycerides / blood


  • Blood Glucose
  • Dietary Fats
  • Insulin
  • Leptin
  • Tea
  • Triglycerides
  • Cholesterol
  • Acyl-CoA Dehydrogenase
  • Acetyl-CoA C-Acyltransferase
  • Carnitine O-Palmitoyltransferase