Uruburuella Testudinis Sp. Nov., Isolated From Tortoise (Testudo)

Int J Syst Evol Microbiol. 2015 Apr;65(Pt 4):1251-1255. doi: 10.1099/ijs.0.000089. Epub 2015 Jan 29.

Abstract

A polyphasic taxonomic analysis was carried out on 11 uncommon Gram-stain-negative, non-motile, catalase- and oxidase-positive, but indole-negative, bacterial strains isolated from tortoises. Phenotypically and genetically they represented a homogeneous group of organisms most closely related to, but distinct from, Uruburuella suis. In a reconstructed 16S rRNA gene tree they clustered on a monophyletic branch next to U. suis with gene similarities between strains of 99.5-100%, and of up to 98.2% with U. suis . DNA-DNA hybridization indicated the organisms represented a novel species with only 40% DNA-DNA similarity with U. suis . Partial sequencing of rpoB resulted in two subclusters confirming the 16S rRNA gene phylogeny; both genes allowed clear separation and identification of the novel species. Furthermore, they could be unambiguously identified by matrix-assisted laser desorption ionization time-of-flight MS, where, again, they formed a highly homogeneous cluster separate from U. suis and other members of the family Neisseriaceae . The major fatty acids were C(16 : 0) and summed feature C(16 : 1)ω7c/iso-C(15 : 0) 2-OH. The DNA G+C content was 54.4 mol%. Based on phenotypic and genetic data we propose classifying these organisms as representatives of a novel species named Uruburuella testudinis sp. nov. The type strain is 07_OD624(T) ( = DSM 26510(T) = CCUG 63373(T)).

MeSH terms

  • Animals
  • Bacterial Typing Techniques
  • Base Composition
  • DNA, Bacterial / genetics
  • Fatty Acids / chemistry
  • Genes, Bacterial
  • Molecular Sequence Data
  • Neisseriaceae / classification*
  • Neisseriaceae / genetics
  • Neisseriaceae / isolation & purification
  • Nucleic Acid Hybridization
  • Phylogeny*
  • RNA, Ribosomal, 16S / genetics
  • Sequence Analysis, DNA
  • Turtles / microbiology*

Substances

  • DNA, Bacterial
  • Fatty Acids
  • RNA, Ribosomal, 16S

Associated data

  • GENBANK/JX966318
  • GENBANK/JX966319
  • GENBANK/JX966320
  • GENBANK/JX966321
  • GENBANK/JX966322
  • GENBANK/JX966323
  • GENBANK/JX966324
  • GENBANK/JX966325
  • GENBANK/JX966326
  • GENBANK/JX966327
  • GENBANK/JX966328
  • GENBANK/JX966329
  • GENBANK/JX966330
  • GENBANK/JX966331
  • GENBANK/JX966332
  • GENBANK/JX966333
  • GENBANK/JX966334
  • GENBANK/JX966335
  • GENBANK/JX966336
  • GENBANK/JX966337
  • GENBANK/JX966338
  • GENBANK/JX966339
  • GENBANK/KM438028
  • GENBANK/KM438029
  • GENBANK/KM438030