Complete Genome Sequence of Mycobacterium sp. Strain VKM Ac-1817D, Capable of Producing 9α-Hydroxy-androst-4-ene-3,17-dione from Phytosterol

Victoriya Y Shtratnikova; Mikhail I Schelkunov; Dmitry V Dovbnya; Yury A Pekov; Eugeny Y Bragin; Vasily V Ashapkin; Marina V Donova

doi:10.1128/genomeA.01447-14

Complete Genome Sequence of Mycobacterium sp. Strain VKM Ac-1817D, Capable of Producing 9α-Hydroxy-androst-4-ene-3,17-dione from Phytosterol

Genome Announc. 2015 Jan 29;3(1):e01447-14. doi: 10.1128/genomeA.01447-14.

Authors

Victoriya Y Shtratnikova¹, Mikhail I Schelkunov¹, Dmitry V Dovbnya², Yury A Pekov¹, Eugeny Y Bragin², Vasily V Ashapkin³, Marina V Donova⁴

Affiliations

¹ Department of Bioengineering and Bioinformatics, M.V. Lomonosov Moscow State University, Moscow, Russia.
² G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Pushchino, Moscow Region, Russia.
³ A.N. Belozersky Institute of Physico-Chemical Biology, M.V. Lomonosov Moscow State University, Moscow, Russia.
⁴ G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Pushchino, Moscow Region, Russia donova@ibpm.pushchino.ru.

Abstract

Mycobacterium sp. strain VKM Ac-1817D is capable of converting phytosterol into 9α-hydroxy androst-4-ene-3,17-dione (9-OH-AD), which is a valuable intermediate for the steroid pharmaceutical industry. Here, a complete genome sequence of the strain is reported. The genome consists of a single circular 6,324,222-bp chromosome with a G+C content of 66.2% and encodes approximately 6,000 CDSs, 54 tRNAs, and 6 rRNAs.