Separation of CFU-S From Primitive Cells Responsible for Reconstitution of the Bone Marrow Hemopoietic Stem Cell Compartment Following Irradiation: Evidence for a pre-CFU-S Cell

Exp Hematol. 1989 Mar;17(3):263-6.

Abstract

We have studied the in vivo spleen colony-forming ability and marrow repopulating ability of murine bone marrow cells differing in mitochondrial activity. Following centrifugal elutriation the cells were sorted on the basis of rhodamine-123 fluorescence intensity within a predetermined light scatter window. It is shown that a class of hemopoietic stem cells exists that differs from the majority of day-12 spleen colony-forming units (CFU-S) in that it has low mitochondrial activity and a high capacity to generate in time new day-12 CFU-S and cells that rescue recipients from radiation-inflicted death. These data add direct evidence for the identity of pre-CFU-S and CFU-S by physical separation.

MeSH terms

  • Animals
  • Bone Marrow / physiology*
  • Bone Marrow / radiation effects
  • Bone Marrow Transplantation
  • Cell Separation*
  • Flow Cytometry
  • Hematopoietic Stem Cell Transplantation
  • Hematopoietic Stem Cells / classification*
  • Hematopoietic Stem Cells / radiation effects
  • Lethal Dose 50
  • Male
  • Mice
  • Radiation Chimera*
  • Spleen / physiology*
  • Spleen / radiation effects
  • Spleen / transplantation