Draconibacterium filum sp. nov., a new species of the genus of Draconibacterium from sediment of the east coast of the Korean Peninsula

Antonie Van Leeuwenhoek. 2015 Apr;107(4):1049-56. doi: 10.1007/s10482-015-0396-4. Epub 2015 Jan 31.

Abstract

A Gram-stain negative, long rod shaped, facultatively anaerobic bacterium, designated strain F2(T), was isolated from coastal sediment of the Korean Peninsula. Strain F2(T) was found to grow at 10-40 °C (optimum 30 °C), at pH 6.0-8.5 (optimum pH 7.5) and at 0.0-8.0 % (w/v) NaCl (optimum 3.0 %). Phylogenetic analysis of the 16S rRNA gene sequence showed that strain F2(T) is closely related to Draconibacterium orientale FH5(T) (with 97.9 % 16S rRNA gene similarity) of the family Prolixibacteraceae of the phylum Bacteroidetes. The major isoprenoid quinone was identified as MK-7 and the main fatty acids as iso-C15:0 (24.1 %), anteiso-C15:0 (15.4 %), C16:0 (10.7 %), iso-C17:0 3-OH (7.6 %) and iso-C16:0 3-OH (5.9 %). The major polar lipids were identified as phosphatidylethanolamine, phosphatidylinositol and four unidentified polar lipids. The genomic DNA G+C content of strain F2(T) was determined to be 44.7 mol% and the DNA-DNA relatedness of strain F2(T) with D. orientale DSM 25947(T) was 34.6 ± 4.3 %. Nitrate reduction capability and cell morphology of strain F2(T) are distinct from those of the closest relative, D. orientale DSM 25947(T). Based on these properties, we propose strain F2(T) represents a novel species within the genus Draconibacterium, with the name Draconibacterium filum sp. nov. The type strain of D. filum is F2(T) (=KCTC 32486(T) = JCM 19986(T)).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aerobiosis
  • Anaerobiosis
  • Bacterial Typing Techniques
  • Bacteroidetes / classification*
  • Bacteroidetes / genetics
  • Bacteroidetes / isolation & purification*
  • Bacteroidetes / physiology
  • Base Composition
  • Cluster Analysis
  • Cytosol / chemistry
  • DNA, Bacterial / chemistry
  • DNA, Bacterial / genetics
  • DNA, Ribosomal / chemistry
  • DNA, Ribosomal / genetics
  • Fatty Acids / analysis
  • Geologic Sediments*
  • Hydrogen-Ion Concentration
  • Korea
  • Microscopy
  • Molecular Sequence Data
  • Nitrates / metabolism
  • Nucleic Acid Hybridization
  • Oxidation-Reduction
  • Phospholipids / analysis
  • Phylogeny
  • Quinones / analysis
  • RNA, Ribosomal, 16S / genetics
  • Sequence Analysis, DNA
  • Sodium Chloride / metabolism
  • Temperature

Substances

  • DNA, Bacterial
  • DNA, Ribosomal
  • Fatty Acids
  • Nitrates
  • Phospholipids
  • Quinones
  • RNA, Ribosomal, 16S
  • Sodium Chloride

Associated data

  • GENBANK/KF933837