Galanin is an autocrine myelin and oligodendrocyte trophic signal induced by leukemia inhibitory factor

Glia. 2015 Jun;63(6):1005-20. doi: 10.1002/glia.22798. Epub 2015 Jan 29.


In order to further investigate the molecular mechanisms that regulate oligodendrocyte (OC) survival, we utilized microarrays to characterize changes in OC gene expression after exposure to the cytokines neurotrophin3, insulin, or leukemia inhibitory factor (LIF) in vitro. We identified and validated the induction and secretion of the neuropeptide galanin in OCs, specifically in response to LIF. We next established that galanin is an OC survival factor and showed that autocrine or paracrine galanin secretion mediates LIF-induced OC survival in vitro. We also revealed that galanin is up-regulated in OCs in the cuprizone model of central demyelination, and that oligodendroglial galanin expression is significantly regulated by endogenous LIF in this context. We also showed that knock-out of galanin reduces OC survival and exacerbates callosal demyelination in the cuprizone model. These findings suggest a potential role for the use of galanin agonists in the treatment of human demyelinating diseases.

Keywords: galanin; leukemia inhibitory factor; oligodendrocytes.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Astrocytes / pathology
  • Astrocytes / physiology
  • Brain / pathology
  • Brain / physiopathology
  • Cell Survival / physiology
  • Cells, Cultured
  • Cuprizone
  • Demyelinating Diseases / pathology
  • Demyelinating Diseases / physiopathology
  • Disease Models, Animal
  • Galanin / genetics
  • Galanin / metabolism*
  • Gene Expression
  • Leukemia Inhibitory Factor / metabolism*
  • MAP Kinase Signaling System / physiology
  • Mice, Inbred C57BL
  • Mice, Knockout
  • Myelin Sheath / pathology
  • Myelin Sheath / physiology*
  • Neural Stem Cells / pathology
  • Neural Stem Cells / physiology
  • Oligodendroglia / pathology
  • Oligodendroglia / physiology*
  • Optic Nerve / pathology
  • Optic Nerve / physiology
  • RNA, Messenger / metabolism
  • Rats, Sprague-Dawley


  • Leukemia Inhibitory Factor
  • RNA, Messenger
  • Cuprizone
  • Galanin