Activity attenuates skeletal muscle fiber damage after ischemia and reperfusion

Muscle Nerve. 2015 Oct;52(4):640-8. doi: 10.1002/mus.24581. Epub 2015 Aug 14.


Introduction: In this investigation we aimed to determine whether: (1) physical activity protects rat skeletal muscle from ischemia/reperfusion (I/R) injury; and (2) continued activity after I/R improves the rate of healing.

Methods: Rats were divided into sedentary or active (voluntary wheel running) groups. Active rats ran for 4 weeks before I/R or 4 weeks before plus 4 weeks after I/R.

Results: Activity before I/R resulted in 73.2% less muscle damage (Evans blue dye inclusion). Sedentary and active rats had a similar decline in neural-evoked (∼ 99%) and directly stimulated (∼ 70%) in vivo muscle torque, and a similar reduction in junctophilin 1. Active rats produced 19% and 15% greater neural-evoked torque compared with sedentary rats at 14 and 28 days postinjury, respectively, although the rate of recovery appeared similar.

Conclusions: Activity protects against long-term muscle damage, but not short-term neural injury or excitation-contraction uncoupling. Continued activity neither accelerates nor hinders the rate of functional recovery.

Keywords: exercise; junctophilin 1; muscle injury; rats; trauma.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Animals
  • Body Weight
  • Disease Models, Animal
  • Ischemia / complications*
  • Ischemia / pathology
  • Isometric Contraction / physiology
  • Male
  • Muscle Fibers, Skeletal / pathology*
  • Muscle Fibers, Skeletal / physiology*
  • Muscular Diseases / etiology*
  • Muscular Diseases / pathology*
  • Physical Conditioning, Animal
  • Rats
  • Rats, Inbred Lew
  • Regeneration
  • Reperfusion Injury / complications*
  • Reperfusion Injury / pathology
  • Running
  • Statistics, Nonparametric
  • Torque