Microfluidic Single-Cell Analysis with Affinity Beads

Small. 2015 Jun 10;11(22):2607-13. doi: 10.1002/smll.201402650. Epub 2015 Jan 29.

Abstract

A micrometer-sized affinity bead (red) is (i) taken up into a cell by phagocytosis, (ii) photochemically released from phagosomes, (iii) optically trapped by the cell, and (iv) isolated by cell lysis for subsequent analysis of captured intracellular analyte (green).

Keywords: affinity beads; microfluidics; optical tweezers; photochemical internalization; single-cell analysis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Separation / methods*
  • Flow Cytometry / methods*
  • HEK293 Cells
  • Humans
  • Immunoassay / methods*
  • Microfluidics / methods*
  • Optical Tweezers*