Molecular investigation of 2013 dengue Fever outbreak from delhi, India

Nazia Afreen; Farah Deeba; Irshad Naqvi; Mohammad Shareef; Anwar Ahmed; Shobha Broor; Shama Parveen

doi:10.1371/currents.outbreaks.0411252a8b82aa933f6540abb54a855f

Molecular investigation of 2013 dengue Fever outbreak from delhi, India

PLoS Curr. 2014 Sep 2:6:ecurrents.outbreaks.0411252a8b82aa933f6540abb54a855f. doi: 10.1371/currents.outbreaks.0411252a8b82aa933f6540abb54a855f.

Authors

Nazia Afreen¹, Farah Deeba¹, Irshad Naqvi², Mohammad Shareef², Anwar Ahmed³, Shobha Broor⁴, Shama Parveen¹

Affiliations

¹ Centre for Interdisciplinary Research in Basic Sciences, Jamia Millia Islamia, New Delhi, India.
² Dr. M. A. Ansari Health Centre, Jamia Millia Islamia, New Delhi, India.
³ Department of Biochemistry, College of Science, King Saud University, Riyadh, Kingdom of Saudi Arabia.
⁴ Department of Microbiology, Jamia Hamdard, New Delhi, India.

PMID: 25642359
PMCID: PMC4169351
DOI: 10.1371/currents.outbreaks.0411252a8b82aa933f6540abb54a855f

Abstract

Dengue fever is a self-limiting, acute febrile disease which may aggravate to haemorrhage, plasma leakage and organ impairment in small number of cases. An outbreak of dengue fever occurred in Delhi, India after rainy season in the year 2013. Dengue virus specific RT-PCR was carried out on 378 suspected blood samples that were collected during the outbreak. Dengue virus was detected in 71% samples with highest number of patients infected by DENV-2 (86%) followed by DENV-1 (19 %) and DENV-3 (8%). Co-infection with more than one DENV serotype was detected in 14% samples. Twenty nine DENV strains (10 DENV-1, 12 DENV-2 and 7 DENV-3) were sequenced for partial envelope protein gene. Phylogenetic analysis grouped DENV-1 strains in the American African genotype, DENV-2 strains in the Cosmopolitan genotype and DENV-3 in Genotype III. We report the serotype distribution, circulating genotypes and partial envelope protein gene sequence of 29 DENV strains detected during 2013 outbreak in Delhi, India.

Grants and funding

The study was funded by University Grants Commission, Government of India. The URL of the funder’s website is http://www.ugc.ac.in. The grant number is 41-1287/2012 (SR).The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.