Reporters for sensitive and quantitative measurement of auxin response

Nat Methods. 2015 Mar;12(3):207-10, 2 p following 210. doi: 10.1038/nmeth.3279. Epub 2015 Feb 2.

Abstract

The visualization of hormonal signaling input and output is key to understanding how multicellular development is regulated. The plant signaling molecule auxin triggers many growth and developmental responses, but current tools lack the sensitivity or precision to visualize these. We developed a set of fluorescent reporters that allow sensitive and semiquantitative readout of auxin responses at cellular resolution in Arabidopsis thaliana. These generic tools are suitable for any transformable plant species.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Arabidopsis / drug effects
  • Arabidopsis / genetics*
  • Arabidopsis / growth & development
  • Gene Expression Regulation, Plant
  • Genes, Reporter*
  • Green Fluorescent Proteins / genetics
  • Green Fluorescent Proteins / metabolism
  • Indoleacetic Acids / metabolism*
  • Indoleacetic Acids / pharmacology
  • Luminescent Proteins / genetics
  • Luminescent Proteins / metabolism
  • Molecular Imaging / methods
  • Plant Roots / genetics
  • Plant Roots / metabolism
  • Plants, Genetically Modified
  • Promoter Regions, Genetic
  • Receptors, TNF-Related Apoptosis-Inducing Ligand / genetics
  • Response Elements / genetics*
  • Signal Transduction / genetics

Substances

  • Indoleacetic Acids
  • Luminescent Proteins
  • Receptors, TNF-Related Apoptosis-Inducing Ligand
  • Green Fluorescent Proteins