Human CD2 3'-flanking sequences confer high-level, T cell-specific, position-independent gene expression in transgenic mice

Cell. 1989 Mar 24;56(6):979-86. doi: 10.1016/0092-8674(89)90631-4.

Abstract

We have localized a set of T cell-specific DNAase I hypersensitive sites in the 3'-flanking region of the human CD2 gene. A 5.5 kb BamHI-XbaI fragment containing these DNAase I hypersensitive sites conferred efficient, copy number-dependent, T cell-specific expression of a linked human CD2 minigene, independent of the position of integration in the transgenic mouse genome. When linked to the mouse Thy-1.1 gene or the human beta-globin gene, this fragment conferred the same T cell-specific expression, independent of its orientation. These results suggest that this flanking region is both necessary and sufficient for full tissue-specific activation of homologous and heterologous genes in transgenic mice.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antigens, Differentiation, T-Lymphocyte / analysis
  • Antigens, Differentiation, T-Lymphocyte / genetics*
  • Antigens, Surface / genetics
  • Base Sequence
  • CD2 Antigens
  • Chromosome Mapping
  • Deoxyribonuclease I / metabolism
  • Gene Expression Regulation
  • Globins / genetics
  • Humans
  • Mice
  • Mice, Transgenic / genetics*
  • Receptors, Immunologic / analysis
  • Receptors, Immunologic / genetics*
  • T-Lymphocytes / immunology*
  • Thy-1 Antigens

Substances

  • Antigens, Differentiation, T-Lymphocyte
  • Antigens, Surface
  • CD2 Antigens
  • Receptors, Immunologic
  • Thy-1 Antigens
  • Globins
  • Deoxyribonuclease I