Dynamic subcellular localization of aquaporin-7 in white adipocytes

Takayuki Miyauchi; Hiroyuki Yamamoto; Yoichiro Abe; Go J Yoshida; Aleksandra Rojek; Eisei Sohara; Shinichi Uchida; Søren Nielsen; Masato Yasui

doi:10.1016/j.febslet.2015.01.025

Dynamic subcellular localization of aquaporin-7 in white adipocytes

FEBS Lett. 2015 Feb 27;589(5):608-14. doi: 10.1016/j.febslet.2015.01.025. Epub 2015 Jan 30.

Authors

Takayuki Miyauchi¹, Hiroyuki Yamamoto², Yoichiro Abe², Go J Yoshida², Aleksandra Rojek³, Eisei Sohara⁴, Shinichi Uchida⁴, Søren Nielsen³, Masato Yasui⁵

Affiliations

¹ Department of Pharmacology, School of Medicine, Keio University, 35 Shinanomachi, Shinjuku, Tokyo 160-8582, Japan. Electronic address: miyauchi@z3.keio.jp.
² Department of Pharmacology, School of Medicine, Keio University, 35 Shinanomachi, Shinjuku, Tokyo 160-8582, Japan.
³ Department of Health Science and Technology, Aalborg University, Fredrik Bajersvej 7, 9220 Aalborg, Denmark.
⁴ Department of Nephrology, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, Yushima, Bunkyo, Tokyo 113-8519, Japan.
⁵ Department of Pharmacology, School of Medicine, Keio University, 35 Shinanomachi, Shinjuku, Tokyo 160-8582, Japan. Electronic address: myasui@a3.keio.jp.

PMID: 25643985
DOI: 10.1016/j.febslet.2015.01.025

Abstract

Aquaporin-7 (AQP7) is expressed in adipose tissue, permeated by water and glycerol, and is involved in lipid metabolism. AQP7-null mice develop obesity, insulin resistance, and adipocyte hypertrophy. Here, we show that AQP7 is expressed in adipocyte plasma membranes, and is re-localized to intracellular membranes in response to catecholamine in mouse white adipose tissue. We found that internalization of AQP7 was induced by PKA activation and comparative gene identification 58 (CGI-58). This relocation was confirmed by functional studies in 3T3-L1 adipocytes. Collectively, these results suggest that AQP7 makes several contributions to adipocyte metabolism, in both cortical and intracellular membranes.

Keywords: Adipocyte; Aquaporin-7; Comparative gene identification 58; Lipolysis.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

1-Acylglycerol-3-Phosphate O-Acyltransferase / genetics
1-Acylglycerol-3-Phosphate O-Acyltransferase / metabolism
3T3-L1 Cells
Adipocytes, White / metabolism*
Animals
Aquaporins / metabolism*
Cyclic AMP-Dependent Protein Kinases / genetics
Cyclic AMP-Dependent Protein Kinases / metabolism
Immunohistochemistry
Mice
Protein Transport

Substances

Aquaporins
1-Acylglycerol-3-Phosphate O-Acyltransferase
Abhd5 protein, mouse
Cyclic AMP-Dependent Protein Kinases