Skip to main page content
Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2015 May;1851(5):566-76.
doi: 10.1016/j.bbalip.2015.01.013. Epub 2015 Jan 31.

Caloric Restriction and Intermittent Fasting Alter Hepatic Lipid Droplet Proteome and Diacylglycerol Species and Prevent Diabetes in NZO Mice

Affiliations
Free article

Caloric Restriction and Intermittent Fasting Alter Hepatic Lipid Droplet Proteome and Diacylglycerol Species and Prevent Diabetes in NZO Mice

Christian Baumeier et al. Biochim Biophys Acta. .
Free article

Abstract

Caloric restriction and intermittent fasting are known to improve glucose homeostasis and insulin resistance in several species including humans. The aim of this study was to unravel potential mechanisms by which these interventions improve insulin sensitivity and protect from type 2 diabetes. Diabetes-susceptible New Zealand Obese mice were either 10% calorie restricted (CR) or fasted every other day (IF), and compared to ad libitum (AL) fed control mice. AL mice showed a diabetes prevalence of 43%, whereas mice under CR and IF were completely protected against hyperglycemia. Proteomic analysis of hepatic lipid droplets revealed significantly higher levels of PSMD9 (co-activator Bridge-1), MIF (macrophage migration inhibitor factor), TCEB2 (transcription elongation factor B (SIII), polypeptide 2), ACY1 (aminoacylase 1) and FABP5 (fatty acid binding protein 5), and a marked reduction of GSTA3 (glutathione S-transferase alpha 3) in samples of CR and IF mice. In addition, accumulation of diacylglycerols (DAGs) was significantly reduced in livers of IF mice (P=0.045) while CR mice showed a similar tendency (P=0.062). In particular, 9 DAG species were significantly reduced in response to IF, of which DAG-40:4 and DAG-40:7 also showed significant effects after CR. This was associated with a decreased PKCε activation and might explain the improved insulin sensitivity. In conclusion, our data indicate that protection against diabetes upon caloric restriction and intermittent fasting associates with a modulation of lipid droplet protein composition and reduction of intracellular DAG species.

Keywords: Caloric restriction; Diacylglycerol; Fatty acid oxidation; Insulin resistance; Intermittent fasting; Lipid droplet proteome.

Similar articles

See all similar articles

Cited by 27 articles

See all "Cited by" articles

Publication types

MeSH terms

LinkOut - more resources

Feedback