NADPH Oxidase 4 Is a Critical Mediator in Ataxia Telangiectasia Disease

Proc Natl Acad Sci U S A. 2015 Feb 17;112(7):2121-6. doi: 10.1073/pnas.1418139112. Epub 2015 Feb 2.

Abstract

Ataxia telangiectasia (A-T), a rare autosomal recessive disorder characterized by progressive cerebellar degeneration and a greatly increased incidence of cancer among other symptoms, is caused by a defective or missing ataxia telangiectasia mutated (ATM) gene. The ATM protein has roles in DNA repair and in the regulation of reactive oxygen species (ROS). Here, we provide, to our knowledge, the first evidence that NADPH oxidase 4 (NOX4) is involved in manifesting A-T disease. We showed that NOX4 expression levels are higher in A-T cells, and that ATM inhibition leads to increased NOX4 expression in normal cells. A-T cells exhibit elevated levels of oxidative DNA damage, DNA double-strand breaks and replicative senescence, all of which are partially abrogated by down-regulation of NOX4 with siRNA. Sections of degenerating cerebelli from A-T patients revealed elevated NOX4 levels. ATM-null mice exhibit A-T disease but they die from cancer before the neurological symptoms are manifested. Injecting Atm-null mice with fulvene-5, a specific inhibitor of NOX4 and NADPH oxidase 2 (NOX2), decreased their elevated cancer incidence to that of the controls. We conclude that, in A-T disease in humans and mice, NOX4 may be critical mediator and targeting it will open up new avenues for therapeutic intervention in neurodegeneration.

Keywords: DNA damage; NOX4; ROS; ataxia telangiectasia; neurodegeneration.

Publication types

  • Research Support, N.I.H., Intramural

MeSH terms

  • Adult
  • Animals
  • Ataxia Telangiectasia / enzymology*
  • Ataxia Telangiectasia / pathology
  • DNA Damage
  • DNA Replication
  • Female
  • Humans
  • Male
  • Mice
  • Middle Aged
  • NADPH Oxidase 4
  • NADPH Oxidases / metabolism*
  • Young Adult

Substances

  • NADPH Oxidase 4
  • NADPH Oxidases
  • NOX4 protein, human