Bacteriophage Transduction in Staphylococcus aureus: Broth-Based Method

Methods Mol Biol. 2016;1373:63-8. doi: 10.1007/7651_2014_185.

Abstract

The ability to move DNA between Staphylococcus strains is essential for the genetic manipulation of this bacterium. Often in the Staphylococci, this is accomplished through transduction using generalized transducing phage and can be performed in different ways and therefore the presence of two transduction procedures in this book. The following protocol is a relatively easy-to-perform, broth-based procedure that we have used extensively to move both plasmids and chromosomal fragments between strains of Staphylococcus aureus.

Keywords: Bacteriophage; Transduction; phi11; phi80α.

MeSH terms

  • Bacteriophages / genetics*
  • Humans
  • Plasmids / genetics
  • Staphylococcal Infections / genetics*
  • Staphylococcal Infections / microbiology
  • Staphylococcus aureus / genetics*
  • Transduction, Genetic / methods*