Upregulation of MicroRNA-126 Contributes to Endothelial Progenitor Cell Function in Deep Vein Thrombosis via Its Target PIK3R2

J Cell Biochem. 2015 Aug;116(8):1613-23. doi: 10.1002/jcb.25115.

Abstract

Deep vein thrombosis (DVT) is a common complication of surgery. Endothelial progenitor cells (EPCs) are recruited into resolving venous thrombi. In this report, we investigated the effects of miR-126 on EPCs function and venous thrombus resolution. We demonstrated that overexpression of miR-126 enhanced EPCs' migration and tubulogenic activity in vitro, and promoted EPCs' homing and thrombus resolving in vivo. Moreover, we identified that miR-126 directly targeted PIK3R2 and affected PI3K/Akt signaling axis. Overall, our findings demonstrated that miR-126 promoted EPCs function through suppressing PIK3R2 expression and modulation of miR-126 may represent a potential therapeutic intervention for treating DVT.

Keywords: EPCs; HOMING; MIGRATION; THROMBUS RESOLUTION; miR-126.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 3' Untranslated Regions
  • Animals
  • Cell Movement
  • Class Ia Phosphatidylinositol 3-Kinase
  • Disease Models, Animal
  • Endothelial Progenitor Cells / physiology*
  • Male
  • MicroRNAs / genetics*
  • Phosphatidylinositol 3-Kinases / genetics*
  • Phosphatidylinositol 3-Kinases / metabolism
  • Rats
  • Rats, Sprague-Dawley
  • Signal Transduction
  • Up-Regulation*
  • Venous Thrombosis / genetics*
  • Venous Thrombosis / metabolism

Substances

  • 3' Untranslated Regions
  • MIRN126 microRNA, rat
  • MicroRNAs
  • PIK3R2 protein, rat
  • Phosphatidylinositol 3-Kinases
  • Class Ia Phosphatidylinositol 3-Kinase