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. 2015 Feb 5;518(7537):27-9.

doi: 10.1038/518027a.

Reproducibility: Standardize antibodies used in research

Andrew Bradbury¹, Andreas Plückthun²

Affiliations

Affiliations

¹ Bioscience Division, Los Alamos National Laboratory, Los Alamos, New Mexico, USA.
² Biochemistry Department of the University of Zurich, Switzerland.

PMID: 25652980
DOI: 10.1038/518027a

Reproducibility: Standardize antibodies used in research

Andrew Bradbury et al. Nature. 2015.

. 2015 Feb 5;518(7537):27-9.

doi: 10.1038/518027a.

Authors

Andrew Bradbury¹, Andreas Plückthun²

Affiliations

¹ Bioscience Division, Los Alamos National Laboratory, Los Alamos, New Mexico, USA.
² Biochemistry Department of the University of Zurich, Switzerland.

PMID: 25652980
DOI: 10.1038/518027a

No abstract available

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Comment in

Antibodies: Validate recombinants too.
Freedman LP. Freedman LP. Nature. 2015 Feb 26;518(7540):483. doi: 10.1038/518483c. Nature. 2015. PMID: 25719653 No abstract available.
Antibodies: The solution is validation.
Polakiewicz RD. Polakiewicz RD. Nature. 2015 Feb 26;518(7540):483. doi: 10.1038/518483b. Nature. 2015. PMID: 25719656 No abstract available.
Reproducibility: bypass animals for antibody production.
Gray AC, Bradbury A, Dübel S, Knappik A, Plückthun A, Borrebaeck CAK. Gray AC, et al. Nature. 2020 May;581(7808):262. doi: 10.1038/d41586-020-01474-7. Nature. 2020. PMID: 32415238 No abstract available.

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References

1. Proteomics. 2011 Dec;11(23):4578-82 - PubMed
1. Biotechniques. 2010 Mar;48(3):197-209 - PubMed
1. PeerJ. 2013 Sep 05;1:e148 - PubMed
1. Cold Spring Harb Perspect Biol. 2012 Mar 01;4(3): - PubMed
1. Mol Cell Proteomics. 2008 Oct;7(10):2019-27 - PubMed

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