d-Amino acids do not inhibit biofilm formation in Staphylococcus aureus

PLoS One. 2015 Feb 6;10(2):e0117613. doi: 10.1371/journal.pone.0117613. eCollection 2015.


Bacteria can either exist in the planktonic (free floating) state or in the biofilm (encased within an organic framework) state. Bacteria biofilms cause industrial concerns and medical complications and there has been a great deal of interest in the discovery of small molecule agents that can inhibit the formation of biofilms or disperse existing structures. Herein we show that, contrary to previously published reports, d-amino acids do not inhibit biofilm formation of Bacillus subtilis (B. subtilis), Staphylococcus aureus (S. aureus), and Staphylococcus epidermis (S. epidermis) at millimolar concentrations. We evaluated a diverse set of natural and unnatural d-amino acids and observed no activity from these compounds in inhibiting biofilm formation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acids / chemistry
  • Amino Acids / pharmacology*
  • Bacillus subtilis / drug effects
  • Bacillus subtilis / physiology
  • Biofilms / drug effects*
  • Biofilms / growth & development
  • Molecular Structure
  • Phenylalanine / chemistry
  • Phenylalanine / pharmacology
  • Proline / chemistry
  • Proline / pharmacology
  • Staphylococcus aureus / drug effects*
  • Staphylococcus aureus / physiology
  • Staphylococcus epidermidis / drug effects
  • Staphylococcus epidermidis / physiology
  • Stereoisomerism
  • Tryptophan / chemistry
  • Tryptophan / pharmacology
  • Tyrosine / chemistry
  • Tyrosine / pharmacology


  • Amino Acids
  • Tyrosine
  • Phenylalanine
  • Tryptophan
  • Proline

Grant support

This work was supported by: Lehigh University start up. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.