Genetic Variants of DICE1/INTS6 in German Prostate Cancer Families with Linkage to 13q14

Urol Int. 2015;95(4):386-9. doi: 10.1159/000366229. Epub 2015 Jan 31.

Abstract

Introduction: Prostate cancer is the most frequent malignancy found to occur in Caucasian men, but its genetic basis remains elusive. A prostate cancer-susceptibility locus has been identified on chromosome 13q14. The tumour suppressor gene deleted in cancer cells 1 (DICE1/INTS6) is located within this interval on 13q14.3.

Materials and methods: We performed mutation analysis of the DICE1/INTS6 gene in thirteen German prostate cancer families.

Results and conclusion: None of the patients harboured DICE1 mutations, and similar frequencies of the previously identified 13 bp deletion polymorphism in the DICE1 promoter were observed in the familial prostate cancer patients as compared with sporadic prostate cancer patients and controls. However, in one family with three affected brothers, the variations c.1215A>C (p.T405T) in exon 10 and c.2568A>G (p.S856S) in exon 17 were detected in a heterozygous pattern. In sporadic prostate cancer patients, variant c.2568A>G (p.S856S) was detected in 10/325 (3.08%) compared with 5/207 (2.42%) control samples (p > 0.05). We conclude that DICE1 appears to be involved in prostate cancer progression rather than in the initiation of prostate cancer.

MeSH terms

  • Aged
  • DNA Mutational Analysis
  • DNA, Neoplasm / analysis*
  • Family*
  • Gene Frequency
  • Genetic Predisposition to Disease / epidemiology*
  • Genetic Predisposition to Disease / genetics
  • Genetic Variation*
  • Germany / epidemiology
  • Humans
  • Male
  • Middle Aged
  • Mutation
  • Polymorphism, Genetic
  • Prostatic Neoplasms / epidemiology
  • Prostatic Neoplasms / genetics*
  • Prostatic Neoplasms / metabolism
  • RNA-Binding Proteins
  • Ribosomal Proteins / genetics*
  • Ribosomal Proteins / metabolism
  • Tumor Suppressor Proteins / genetics*
  • Tumor Suppressor Proteins / metabolism

Substances

  • DNA, Neoplasm
  • INTS6 protein, human
  • RNA-Binding Proteins
  • Ribosomal Proteins
  • Tumor Suppressor Proteins