Sandwich enzyme-linked immunosorbent assay for the quantification of human serum albumin fragment 408-423 in bodily fluids

Anal Biochem. 2015 May 1;476:29-35. doi: 10.1016/j.ab.2015.01.023. Epub 2015 Feb 4.

Abstract

Urinary levels of human serum albumin (hSA) fragment 408-423 have been proposed to represent an early marker for graft-versus-host disease (GvHD) and chronic kidney diseases. Here, we developed an enzyme-linked immunosorbent assay (ELISA) for the quantification of hSA(408-423). The sandwich ELISA has a detection limit of 0.5ng/ml and is highly specific for hSA(408-423) because it does not cross-react with other albumin fragments or the full-length precursor. This ELISA allows rapid and convenient quantification of hSA(408-423) in bodily fluids, further clarifying the prognostic and diagnostic value of this peptide in GvHD, kidney disease, and other disorders.

Keywords: Biomarker; ELISA; Graft-versus-host disease; Human serum albumin; Kidney disease; Peptide.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Albumins / chemistry*
  • Enzyme-Linked Immunosorbent Assay / methods*
  • Graft vs Host Disease / metabolism*
  • Humans
  • Kidney Diseases / metabolism
  • Serum Albumin / metabolism*

Substances

  • Albumins
  • Serum Albumin