Lung inflation with hydrogen during the cold ischemia phase decreases lung graft injury in rats

Exp Biol Med (Maywood). 2015 Sep;240(9):1214-22. doi: 10.1177/1535370214563895. Epub 2015 Feb 7.

Abstract

Hydrogen has antioxidant and anti-inflammatory effects on lung ischemia-reperfusion injury when it is inhaled by donor or/and recipient. This study examined the effects of lung inflation with 3% hydrogen during the cold ischemia phase on lung graft function in rats. The donor lung was inflated with 3% hydrogen, 40% oxygen, and 57% nitrogen at 5 mL/kg, and the gas was replaced every 20 min during the cold ischemia phase for 2 h. In the control group, the donor lung was inflated with 40% oxygen and 60% nitrogen at 5 mL/kg. The recipient was euthanized 2 h after orthotropic lung transplantation. The hydrogen concentration in the donor lung during the cold ischemia phase was 1.99-3%. The oxygenation indices in the arterial blood and pulmonary vein blood were improved in the hydrogen group. The inflammation response indices, including lung W/D ratio, the myeloperoxidase activity in the grafts, and the levels of IL-8 and TNF-α in serum, were significantly lower in the hydrogen group (5.2 ± 0.8, 0.76 ± 0.32 U/g, 340 ± 84 pg/mL, and 405 ± 115 pg/mL, respectively) than those in the control group (6.5 ± 0.7, 1.1 ± 0.5 U/g, 443 ± 94 pg/mL, and 657 ± 96 pg/mL, respectively (P < 0.05), and the oxidative stress indices, including the superoxide dismutase activity and the level of malonaldehyde in lung grafts were improved after hydrogen application. Furthermore, the lung injury score determined by histopathology, the cell apoptotic index, and the caspase-3 protein expression in lung grafts were decreased after hydrogen treatment, and the static pressure-volume curve of lung graft was improved by hydrogen inflation. In conclusion, lung inflation with 3% hydrogen during the cold ischemia phase alleviated lung graft injury and improved graft function.

Keywords: Hydrogen; donor; ischemia reperfusion injury; lung transplantation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antioxidants / administration & dosage
  • Apoptosis / drug effects
  • Cold Ischemia / methods*
  • Hydrogen / administration & dosage*
  • Lung Compliance / drug effects
  • Lung Injury / pathology
  • Lung Injury / physiopathology
  • Lung Injury / prevention & control*
  • Lung Transplantation / methods*
  • Male
  • Organ Preservation / methods*
  • Oxidative Stress / drug effects
  • Oxygen / blood
  • Primary Graft Dysfunction / pathology
  • Primary Graft Dysfunction / physiopathology
  • Primary Graft Dysfunction / prevention & control
  • Rats
  • Rats, Sprague-Dawley
  • Reperfusion Injury / pathology
  • Reperfusion Injury / physiopathology
  • Reperfusion Injury / prevention & control*

Substances

  • Antioxidants
  • Hydrogen
  • Oxygen