Immunomodulatory effects of the Agaricus blazei Murrill-based mushroom extract AndoSan in patients with multiple myeloma undergoing high dose chemotherapy and autologous stem cell transplantation: a randomized, double blinded clinical study

Biomed Res Int. 2015;2015:718539. doi: 10.1155/2015/718539. Epub 2015 Jan 18.

Abstract

Forty patients with multiple myeloma scheduled to undergo high dose chemotherapy with autologous stem cell support were randomized in a double blinded fashion to receive adjuvant treatment with the mushroom extract AndoSan, containing 82% of Agaricus blazei Murrill (19 patients) or placebo (21 patients). Intake of the study product started on the day of stem cell mobilizing chemotherapy and continued until the end of aplasia after high dose chemotherapy, a period of about seven weeks. Thirty-three patients were evaluable for all study endpoints, while all 40 included patients were evaluable for survival endpoints. In the leukapheresis product harvested after stem cell mobilisation, increased percentages of Treg cells and plasmacytoid dendritic cells were found in patients receiving AndoSan. Also, in this group, a significant increase of serum levels of IL-1ra, IL-5, and IL-7 at the end of treatment was found. Whole genome microarray showed increased expression of immunoglobulin genes, Killer Immunoglobulin Receptor (KIR) genes, and HLA genes in the Agaricus group. Furthermore, AndoSan displayed a concentration dependent antiproliferative effect on mouse myeloma cells in vitro. There were no statistically significant differences in treatment response, overall survival, and time to new treatment. The study was registered with Clinicaltrials.gov NCT00970021.

Publication types

  • Randomized Controlled Trial
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Agaricus / chemistry*
  • Aged
  • Animals
  • Biomarkers, Tumor / metabolism
  • Cell Line, Tumor
  • Cell Membrane / metabolism
  • Cell Proliferation / drug effects
  • Chemokines / metabolism
  • Complex Mixtures
  • Dose-Response Relationship, Drug
  • Double-Blind Method
  • Female
  • Gene Expression Regulation
  • Hematopoietic Stem Cell Transplantation*
  • Humans
  • Immunologic Factors / pharmacology
  • Immunologic Factors / therapeutic use*
  • Intercellular Signaling Peptides and Proteins / metabolism
  • Leukapheresis
  • Male
  • Mice
  • Middle Aged
  • Multiple Myeloma / drug therapy*
  • Multiple Myeloma / genetics
  • Signal Transduction / genetics
  • Survival Analysis
  • Time Factors
  • Tissue Extracts / pharmacology
  • Tissue Extracts / therapeutic use*
  • Transplantation, Autologous
  • Treatment Outcome

Substances

  • AndoSan
  • Biomarkers, Tumor
  • Chemokines
  • Complex Mixtures
  • Immunologic Factors
  • Intercellular Signaling Peptides and Proteins
  • Tissue Extracts

Associated data

  • ClinicalTrials.gov/NCT00970021