Behaviour of four different B16 murine melanoma cell sublines: C57BL/6J skin

Int J Exp Pathol. 2015 Apr;96(2):73-80. doi: 10.1111/iep.12114. Epub 2015 Feb 9.


Transplantable murine melanomas are well-established models for the study of experimental cancer therapies. The aim of this study was to explore the behaviour of four different B16 murine melanoma cell sublines after inoculation into C57BL/6J mice; and, more specifically to analyse skin changes, with respect to two specific parameters: clinical (tumour volume, melanin amount, erythema) and histological (H & E, S100, VEGF expression). Both non-invasive and invasive analysis showed that B164A5 is the most aggressive melanoma cell line for C57BL/6J's skin, followed by B16F10 and then by diminished aggressive growth pattern by the B16GMCSF and B16FLT3 cell lines.

Keywords: B164A5; B16F10; B16FLT3; B16GMCSF; C57BL/6J.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Biomarkers, Tumor / metabolism
  • Cell Line, Tumor / pathology*
  • Cell Proliferation
  • Cell Transplantation*
  • Disease Models, Animal
  • Female
  • Melanoma / pathology*
  • Mice
  • Mice, Inbred C57BL
  • Neoplasm Invasiveness / pathology
  • Skin / metabolism
  • Skin / pathology*
  • Skin Neoplasms / pathology*
  • Vascular Endothelial Growth Factor A / metabolism


  • Biomarkers, Tumor
  • Vascular Endothelial Growth Factor A