Structure of a yeast 40S-eIF1-eIF1A-eIF3-eIF3j initiation complex

Nat Struct Mol Biol. 2015 Mar;22(3):269-71. doi: 10.1038/nsmb.2963. Epub 2015 Feb 9.


Eukaryotic translation initiation requires cooperative assembly of a large protein complex at the 40S ribosomal subunit. We have resolved a budding yeast initiation complex by cryo-EM, allowing placement of prior structures of eIF1, eIF1A, eIF3a, eIF3b and eIF3c. Our structure highlights differences in initiation-complex binding to the ribosome compared to that of mammalian eIF3, demonstrates a direct contact between eIF3j and eIF1A and reveals the network of interactions between eIF3 subunits.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Binding Sites
  • Cryoelectron Microscopy
  • Eukaryotic Initiation Factor-1 / chemistry*
  • Eukaryotic Initiation Factor-3 / chemistry*
  • Peptide Chain Initiation, Translational
  • Protein Structure, Tertiary
  • Ribosome Subunits, Small, Eukaryotic / chemistry*
  • Saccharomycetales / genetics*


  • Eukaryotic Initiation Factor-1
  • Eukaryotic Initiation Factor-3

Associated data

  • PDB/4UER