Characterization of foot-and-mouth disease viruses (FMDVs) from Ugandan cattle outbreaks during 2012-2013: evidence for circulation of multiple serotypes

PLoS One. 2015 Feb 9;10(2):e0114811. doi: 10.1371/journal.pone.0114811. eCollection 2015.

Abstract

To investigate the foot-and-mouth disease virus (FMDV) serotypes circulating in Uganda's cattle population, both serological and virological analyses of samples from outbreaks that occurred during 2012-2013 were performed. Altogether, 79 sera and 60 oropharyngeal fluid (OP)/tissue/oral swab samples were collected from herds with reported FMD outbreaks in seven different Ugandan districts. Overall, 61/79 (77%) of the cattle sera were positive for antibodies against FMDV by PrioCHECK FMDV NS ELISA and solid phase blocking ELISA detected titres ≥ 80 for serotypes O, SAT 1, SAT 2 and SAT 3 in 41, 45, 30 and 45 of these 61 seropositive samples, respectively. Virus neutralisation tests detected the highest levels of neutralising antibodies (titres ≥ 45) against serotype O in the herds from Kween and Rakai districts, against SAT 1 in the herd from Nwoya district and against SAT 2 in the herds from Kiruhura, Isingiro and Ntungamo districts. The isolation of a SAT 2 FMDV from Isingiro was consistent with the detection of high levels of neutralising antibodies against SAT 2; sequencing (for the VP1 coding region) indicated that this virus belonged to lineage I within this serotype, like the currently used vaccine strain. From the Wakiso district 11 tissue/swab samples were collected; serotype A FMDV, genotype Africa (G-I), was isolated from the epithelial samples. This study shows that within a period of less than one year, FMD outbreaks in Uganda were caused by four different serotypes namely O, A, SAT 1 and SAT 2. Therefore, to enhance the control of FMD in Uganda, there is need for efficient and timely determination of outbreak virus strains/serotypes and vaccine matching. The value of incorporating serotype A antigen into the imported vaccines along with the current serotype O, SAT 1 and SAT 2 strains should be considered.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Cattle
  • Cattle Diseases / epidemiology*
  • Cattle Diseases / virology*
  • Disease Outbreaks / veterinary*
  • Enzyme-Linked Immunosorbent Assay / veterinary
  • Foot-and-Mouth Disease / epidemiology*
  • Foot-and-Mouth Disease / virology*
  • Foot-and-Mouth Disease Virus / classification
  • Foot-and-Mouth Disease Virus / genetics*
  • Molecular Sequence Data
  • Neutralization Tests / veterinary
  • Phylogeny
  • RNA, Viral
  • Serogroup
  • Uganda / epidemiology

Substances

  • RNA, Viral

Associated data

  • GENBANK/KP089984
  • GENBANK/KP089985
  • GENBANK/KP089986
  • GENBANK/KP089987
  • GENBANK/KP089988
  • GENBANK/KP089989
  • GENBANK/KP089990

Grant support

This work was funded by the Danish International Development Agency (DANIDA) under the Transboundary Animal Diseases in East Africa project, DFC no. 10-006KU (HS). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.