The effect of melatonin on the liver of rats exposed to microwave radiation

Bratisl Lek Listy. 2015;116(2):96-100. doi: 10.4149/bll_2015_018.

Abstract

Objectives: We aimed to clarify if melatonin treatment (2 mg/kg i.p.) may favorably impact the liver tissue in rats exposed to microwave radiation. The experiment was performed on 84 six-weeks-old Wistar male rats exposed for 4h a day, for 20, 40 and 60 days, respectively, to microwaves (900 MHz, 100-300 microT, 54-160 V/m). Rats were divided in to four groups: I (control) - rats treated with saline, II (Mel) - rats treated with melatonin, III (MWs) - microwave exposed rats, IV (MWs + Mel) - MWs exposed rats treated with melatonin. We evaluated oxidative stress parameters (malondialdehyde and carbonyl group content), catalase, xanthine oxidase, deoxyribonuclease I and II activity.

Background: Oxidative stress is the key mechanism of the microwave induced tissue injury. Melatonin, a lipophilic indoleamine primarily synthesized and released from the pineal gland is a powerful antioxidant.

Results: Exposure to microwaves caused an increase in malondialdehyde after 40 (p < 0.01), protein carbonyl content after 20 (p < 0.05), catalase (p < 0.05) and xantine oxidase activity (p < 0.05) after 40 days. Increase in deoxyribonuclease I activity was observed after 60 days (p < 0.05), while deoxyribonuclease II activity was unaffected. Melatonin treatment led to malondialdehyde decrease after 40 days (p< 0.05), but surprisingly had no effect on other analyzed parameters.

Conclusion: Melatonin exerts certain antioxidant effects in the liver of rats exposed to microwaves, by diminishing the intensity of lipid peroxidation(Fig. 6, Ref. 32).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antioxidants / pharmacology*
  • Catalase / drug effects
  • Catalase / metabolism
  • Catalase / radiation effects
  • Dose-Response Relationship, Radiation
  • Lipid Peroxidation / drug effects
  • Lipid Peroxidation / radiation effects
  • Liver Diseases / etiology
  • Liver Diseases / prevention & control*
  • Male
  • Malondialdehyde / metabolism
  • Melatonin / pharmacology*
  • Microwaves / adverse effects*
  • Oxidative Stress / drug effects*
  • Radiation Injuries, Experimental / prevention & control*
  • Rats
  • Rats, Wistar
  • Reactive Oxygen Species / metabolism
  • Xanthine Oxidase / drug effects
  • Xanthine Oxidase / metabolism
  • Xanthine Oxidase / radiation effects

Substances

  • Antioxidants
  • Reactive Oxygen Species
  • Malondialdehyde
  • Catalase
  • Xanthine Oxidase
  • Melatonin