Supplementation of apelin increase plasma levels of nesfatin-1 in normal and DOCA-salt hypertensive rats

Bratisl Lek Listy. 2015;116(2):104-8. doi: 10.4149/bll_2015_020.


Aims: We aimed to observe the effects of apelin supplementation on the plasma levels of nesfatin-1 in DOCA-salt hypertensive and normal rats.

Methods: For this purpose, 28 young Wistar albino male rats were divided into four groups; Control (C), Control + Apelin (C+A), Hypertension (HT) and Hypertension + Apelin (HT+A). Hypertension was induced by injection of DOCA-salt (25 mg/kg, s.c.) twice weekly, 4 weeks, whereas intraperitoneal apelin was administered (200 µ for 17 days. Plasma nesfatin-1 and apelin levels were measured with ELISA. Systolic blood pressure was monitored using a tail cuff system. The relationships between plasma nesfatin levels and blood pressure were assessed.

Results: Plasma nesfatin-1 levels was found lower in control animals compared to C+A, HT and HT+A groups (p = 0.002, p = 0.026 and p = 0.011, respectively). Systolic blood pressures were similar in the C and C+A groups, but systolic blood pressures of the HT and HT+A groups was found significantly higher than the C and C+A groups.

Conclusions: In conclusion, apelin administration induced an increment of nesfatin-1 in normal rats and plasma levels of nesfatin-1 increase in DOCA-salt hypertension rats. But apelin addition in hypertension did not cause an extra increase in nesfatin-1 levels. This is the first report to investigate the effect of apelin administration on plasma nesfatin levels of normal and hypertensive rats (Fig. 2, Ref. 44).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Apelin
  • Calcium-Binding Proteins / blood*
  • Calcium-Binding Proteins / drug effects*
  • DNA-Binding Proteins / blood*
  • DNA-Binding Proteins / drug effects*
  • Desoxycorticosterone Acetate
  • Disease Models, Animal
  • Hypertension / blood*
  • Hypertension / chemically induced
  • Hypertension / drug therapy*
  • Intercellular Signaling Peptides and Proteins / administration & dosage
  • Intercellular Signaling Peptides and Proteins / pharmacology*
  • Male
  • Nerve Tissue Proteins / blood*
  • Nerve Tissue Proteins / drug effects*
  • Nucleobindins
  • Random Allocation
  • Rats
  • Rats, Wistar


  • Apelin
  • Apln protein, rat
  • Calcium-Binding Proteins
  • DNA-Binding Proteins
  • Intercellular Signaling Peptides and Proteins
  • Nerve Tissue Proteins
  • Nucb1 protein, rat
  • Nucleobindins
  • Desoxycorticosterone Acetate