Objectives: The aim of this study was to determine the effects of blood contamination and hemostatic agents on shear bond strength (SBS) of brackets and bond failure.
Materials and methods: The study material consisted of 57 freshly extracted human premolar and randomly divided into four groups: Group I, control group (n0 = 14); Group II, contamination with blood (n = 13); Group III, contamination with epinephrine ( n = 14); and Group IV, contamination with Ankaferd blood stopper (ABS) ( n = 16). After the bracket bonding procedure, all bonded teeth thermal cycled in deionized water at 5 ± 2°C to 55 ± 2°C for 500 cycles. SBS was applied using a universal test machine.
Results: According to Kruskal-Wallis test significant differences were found among the groups P < 0.05. Furthermore, significant differences were recorded between groups with Mann-Whitney U statistical test with Bonferroni correction (P = 0.0083).
Conclusions: Examples contaminated with blood showed a statistically significant lower in vitro SBS than those contaminated with epinephrine, ABS, and control groups.
Clinical significance: In impacted tooth surgical operations, blood contamination poses a substantial risk of bond failure in bonding attachments applications to the impacted teeth. Epinephrine and ABS may be used on surgical exposed impacted teeth operation for the prevention of blood contamination.