The role of cyclin synthesis and degradation in the control of maturation promoting factor activity

Nature. 1989 May 25;339(6222):280-6. doi: 10.1038/339280a0.


We show that cyclin plays a pivotal role in the control of mitosis. A proteolysis-resistant mutant of cyclin prevents the inactivation of maturation promoting factor and the exit from mitosis both in vivo and in vitro. We have used a fractionated extract to study the activation of MPF by added cyclin protein.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Cell Cycle*
  • Embryo, Nonmammalian / cytology
  • Embryo, Nonmammalian / physiology
  • Female
  • Growth Substances / metabolism*
  • Kinetics
  • Maturation-Promoting Factor
  • Meiosis
  • Mitosis
  • Nuclear Proteins / biosynthesis
  • Nuclear Proteins / metabolism*
  • Ovum / cytology
  • Proliferating Cell Nuclear Antigen
  • Xenopus


  • Growth Substances
  • Nuclear Proteins
  • Proliferating Cell Nuclear Antigen
  • Maturation-Promoting Factor