Generation of a felinized swine endothelial cell line by expression of feline decay-accelerating factor

PLoS One. 2015 Feb 11;10(2):e0117682. doi: 10.1371/journal.pone.0117682. eCollection 2015.

Abstract

Embryonic stem cell research has facilitated the generation of many cell types for the production of tissues and organs for both humans and companion animals. Because ≥30% of pet cats suffer from chronic kidney disease (CKD), xenotransplantation between pigs and cats has been studied. For a successful pig to cat xenotransplant, the immune reaction must be overcome, especially hyperacute rejection. In this study, we isolated the gene for feline decay-accelerating factor (fDAF), an inhibitor of complement proteins, and transfected a swine endothelial cell line with fDAF to "felinize" the pig cells. These fDAF-expressing cells were resistant to feline serum containing anti-pig antibodies, suggesting that felinized pig cells were resistant to hyperacute rejection. Our results suggest that a "felinized" pig kidney can be generated for the treatment of CKD in cats in the future.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • CD55 Antigens / chemistry
  • CD55 Antigens / genetics*
  • Cat Diseases / surgery
  • Cats
  • Cell Line
  • Clone Cells / metabolism
  • Cloning, Molecular
  • Endothelial Cells / metabolism*
  • Gene Expression
  • Graft Rejection
  • Humans
  • L-Lactate Dehydrogenase / metabolism
  • Mice
  • Molecular Sequence Data
  • Renal Insufficiency, Chronic / veterinary
  • Swine
  • Transfection
  • Transplantation, Heterologous* / adverse effects

Substances

  • CD55 Antigens
  • L-Lactate Dehydrogenase

Grant support

This study was supported by a Grant-in-Aid for Progressive Renal Diseases Research, Research on Intractable Disease, from the Ministry of Health, Labour and Welfare of Japan and a Adaptable and Seamless Technology transfer Program (AS242Z01218N) from Japan Science and Technology Agency. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.