Increase of DC-LAMP+ mature dendritic cell subsets in dermatopathic lymphadenitis of mycosis fungoides

Eur J Dermatol. 2014 Nov-Dec;24(6):670-5. doi: 10.1684/ejd.2014.2437.


Background: Little is known about the immunological milieu of the skin-draining lymph nodes (LNs) in mycosis fungoides (MF).

Objectives: We studied dendritic cell (DC) subsets in the dermatopathic lymphadenitis of MF patients.

Methods: We immunohistochemically examined DC subsets and their distribution in 16 LN samples from 14 patients with MF (N1 LN, eight patients; N2, four; and N3, four), and we compared them with non-metastatic sentinel LNs from eight patients with melanoma.

Results: The number of S-100 protein+ DCs was markedly increased in the LNs from the MF patients and the major component was DC-LAMP+ mature DCs in the outer and paracortex areas, where DC-SIGN+ immature DCs were relatively decreased in proportion. In contrast, DC-SIGN+ cells were relatively increased in proportion compared to DC-LAMP+ cells in the medulla. Although no significant difference was observed in the proportions of CD1a+ or Langerin+ DCs among the N1, N2, and N3 nodes, CD163+ M2-type macrophages were increased in number in the N2 and N3 nodes.

Conclusions: Our observations indicate that mature DCs accumulate in the outer and paracortex areas in dermatopathic lymphadenitis and M2-type macrophages might increase in number during disease progression.

Keywords: DC-LAMP; DC-SIGN; M2-type macrophage; Mycosis fungoides; dendritic cell; dermatopathic lymphadenitis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Aged
  • Antigens, CD / analysis
  • Antigens, Differentiation, Myelomonocytic / analysis
  • Cell Adhesion Molecules / analysis
  • Cell Count
  • Dendritic Cells / chemistry
  • Dendritic Cells / pathology*
  • Female
  • Humans
  • Immunohistochemistry
  • Lectins, C-Type / analysis
  • Lymph Nodes / pathology*
  • Lymphadenitis / etiology
  • Lymphadenitis / pathology*
  • Lysosomal-Associated Membrane Protein 3 / analysis*
  • Macrophages / chemistry
  • Male
  • Melanoma / pathology*
  • Middle Aged
  • Mycosis Fungoides / complications
  • Mycosis Fungoides / pathology*
  • Neoplasm Staging
  • Receptors, Cell Surface / analysis
  • S100 Proteins / analysis
  • Skin Neoplasms / complications
  • Skin Neoplasms / pathology*


  • Antigens, CD
  • Antigens, Differentiation, Myelomonocytic
  • CD163 antigen
  • Cell Adhesion Molecules
  • DC-specific ICAM-3 grabbing nonintegrin
  • Lectins, C-Type
  • Lysosomal-Associated Membrane Protein 3
  • Receptors, Cell Surface
  • S100 Proteins