Effect of analogues of S-adenosylmethionine on in vitro polyadenylation by vesicular stomatitis virus

J Gen Virol. 1989 Mar;70 ( Pt 3):535-42. doi: 10.1099/0022-1317-70-3-535.

Abstract

Other workers have reported that vesicular stomatitis virus makes aberrantly long polyadenylic acid [poly(A)] tracts in the presence of S-adenosylhomocysteine (S-Ado-Hcy). In the work reported in this paper, the effects of various analogues of S-adenosylmethionine (S-Ado-Met) and ATP on polyadenylation in an in vitro transcription system were examined to determine whether S-Ado-Hcy exerted its effect on polyadenylation due to its relationship to S-Ado-Met or to ATP. It appeared that compounds which affected polyadenylation were those which were closely related to S-Ado-Met and that had the same L-aminoacyl side chain [(COOH)-CH(NH)2-CH2-CH2-]; the nature of the substituent at the -S+(CH3)- position of S-Ado-Met was less important. These analogues appeared to compete with S-Ado-Met for a binding site(s). These data support a model whereby compounds binding at an S-Ado-Met-binding site may have allosteric effects by causing or preventing conformational changes which are involved in polyadenylation reactions, perhaps by affecting the rate of polyadenylation or of termination.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Binding Sites / drug effects
  • Binding, Competitive / drug effects
  • Poly A / biosynthesis*
  • RNA, Messenger / biosynthesis*
  • S-Adenosylmethionine / analogs & derivatives*
  • S-Adenosylmethionine / pharmacology
  • Structure-Activity Relationship
  • Transcription, Genetic / drug effects
  • Vesicular stomatitis Indiana virus / drug effects*
  • Vesicular stomatitis Indiana virus / enzymology

Substances

  • RNA, Messenger
  • Poly A
  • S-Adenosylmethionine