Serological diagnosis of salmonella infections by enzyme immunoassay

Lancet. 1989 Jun 24;1(8652):1411-4. doi: 10.1016/s0140-6736(89)90124-4.


An enzyme immunoassay (EIA) for the detection and measurement of serum IgM, IgG, and IgA antibodies to salmonella was developed with commercially available lipopolysaccharides (LPSs) of Salmonella typhimurium and S enteritidis combined as antigen. Of 130 sera from patients with culture-confirmed salmonella infections, 115 (88.5%) were positive in this assay. The classic Widal agglutination test was positive in only 50 (38.5%) cases. This EIA method offers a substantial advance in the serological diagnosis of acute salmonella infections; it detects antibodies to the salmonellae of groups B and D, which constitute about 70% of culture-positive cases of human salmonellosis. Antibodies to other salmonellae are also detected. This EIA is particularly valuable for the detection of salmonella antibodies during post-infectious complications when isolation of the organism is often no longer possible.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acute Disease
  • Adolescent
  • Adult
  • Aged
  • Antibodies, Bacterial / analysis*
  • Child
  • Child, Preschool
  • Enzyme-Linked Immunosorbent Assay / instrumentation
  • Evaluation Studies as Topic
  • Follow-Up Studies
  • Humans
  • Immunoglobulin A / analysis*
  • Immunoglobulin G / analysis*
  • Immunoglobulin M / analysis*
  • Infant
  • Middle Aged
  • Predictive Value of Tests
  • Salmonella / classification
  • Salmonella / immunology*
  • Salmonella Infections / immunology*
  • Salmonella enteritidis / immunology
  • Salmonella typhimurium / immunology


  • Antibodies, Bacterial
  • Immunoglobulin A
  • Immunoglobulin G
  • Immunoglobulin M