Molecular Detection and Characterization of Infectious Laryngotracheitis Virus (Gallid herpesvirus-1) From Clinical Samples of Commercial Poultry Flocks in India

Virusdisease. 2014;25(3):345-9. doi: 10.1007/s13337-014-0206-z. Epub 2014 Apr 1.

Abstract

Although the existence of infectious laryngotracheitis virus (ILTV) in India was first reported in 1964, no reports are available regarding its molecular detection and characterization. The present study was aimed to detect and characterize ILTV from recent respiratory disease complex (RDC) outbreaks of commercial poultry flocks in different parts of the country by using envelope glycoprotein G gene (US4 gene) based PCR and sequencing. A total of thirty two flocks with a history of RDC were investigated. Overall, all the strains/breeds of birds and all ages of birds are equally susceptible and depending on the severity, the clinical signs and gross lesions were varied. Out of 32 flocks investigated 10 were found positive for ILTV infection by PCR. The phylogenetic analyses of eight representative sequences in the present study deciphered that Indian ILT viruses are closely related to chicken embryo origin vaccine strains of Italy, USA, China and Brazil.

Keywords: India; Infectious laryngotracheitis; Molecular epidemiology; PCR detection; Phylogenetic analyses.