Chemical Constituents of Hericium Erinaceum Associated With the Inhibitory Activity Against Cellular Senescence in Human Umbilical Vascular Endothelial Cells

J Enzyme Inhib Med Chem. 2015 Dec;30(6):934-40. doi: 10.3109/14756366.2014.995181. Epub 2015 Feb 13.

Abstract

Hericium erinaceum is an edible and medicinal mushroom widely used in Korea, Japan, and China. On the search for biologically active compounds supporting the medicinal usage, the MeOH extract of the fruiting bodies of H. erinaceum was investigated for its chemical constituents. Six compounds were isolated and identified as hericenone D (1), (22E,24R)-5α,8α-epidioxyergosta-6,22-dien-3β-ol (2), erinacerin B (3), hericenone E (4), hericenone F (5) and isohericerin (6) by comparing their spectroscopic data with previously reported values. The inhibitory effects on adriamycin-induced cellular senescence in human dermal fibroblasts (HDFs) and human umbilical vein endothelial cells (HUVECs) of the isolates (1-6) were studied. Among the isolated compounds, ergosterol peroxide (2) reduced senescence associated β-galactosidase (SA-β-gal) activity increased in HUVECs treated with adriamycin. According to experimental data obtained, the active compound may inspire the development of a new pharmacologically useful substance to be used in the treatment and prevention of age-related diseases.

Keywords: Ergosterol peroxide; Hericium erinaceum; human dermal fibroblasts; human umbilical vein endothelial cells; senescence associated β-galactosidase activity.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Agaricales / chemistry*
  • Benzopyrans / chemistry
  • Benzopyrans / isolation & purification
  • Benzopyrans / pharmacology
  • Cellular Senescence / drug effects*
  • Dose-Response Relationship, Drug
  • Doxorubicin / antagonists & inhibitors
  • Doxorubicin / pharmacology
  • Ergosterol / analogs & derivatives
  • Ergosterol / chemistry
  • Ergosterol / isolation & purification
  • Ergosterol / pharmacology
  • Fibroblasts / drug effects
  • Fibroblasts / enzymology
  • Heterocyclic Compounds, 2-Ring / chemistry
  • Heterocyclic Compounds, 2-Ring / isolation & purification
  • Heterocyclic Compounds, 2-Ring / pharmacology
  • Human Umbilical Vein Endothelial Cells / cytology*
  • Human Umbilical Vein Endothelial Cells / drug effects*
  • Human Umbilical Vein Endothelial Cells / enzymology
  • Humans
  • Indoles / chemistry
  • Indoles / isolation & purification
  • Indoles / pharmacology
  • Molecular Structure
  • Palmitates / chemistry
  • Palmitates / isolation & purification
  • Palmitates / pharmacology
  • Phenols / chemistry
  • Phenols / isolation & purification
  • Phenols / pharmacology
  • Skin / cytology
  • Skin / drug effects
  • Structure-Activity Relationship
  • Terpenes / chemistry
  • Terpenes / isolation & purification
  • Terpenes / pharmacology
  • beta-Galactosidase / antagonists & inhibitors
  • beta-Galactosidase / metabolism

Substances

  • 5alpha,8alpha-epidioxyergosta-6,22-dien-3-beta-ol
  • Benzopyrans
  • Heterocyclic Compounds, 2-Ring
  • Indoles
  • Palmitates
  • Phenols
  • Terpenes
  • erinacerin B
  • hericenone D
  • hericenone E
  • Doxorubicin
  • beta-Galactosidase
  • Ergosterol