Mix.1, a homeobox mRNA inducible by mesoderm inducers, is expressed mostly in the presumptive endodermal cells of Xenopus embryos

Cell. 1989 Jun 16;57(6):965-74. doi: 10.1016/0092-8674(89)90335-8.

Abstract

In frogs, mesoderm presumably derives from presumptive ectoderm by induction under the control of diffusible substances produced by the endoderm. To analyze the early phase of mesoderm induction, I have isolated cDNA copies of mRNAs induced in presumptive ectoderm by mesoderm inducing factor secreted by the XTC cell line. One of the inducible mRNAs encodes a homeodomain-containing protein that is likely to play a regulatory role in development. Mix.1 behaves as an immediate early response to induction, and its kinetics of expression suggest a major role for MBT in the control of inducible gene expression. Unexpectedly, Mix.1 is expressed mostly in the future endoderm, suggesting that endoderm may be formed by induction in a similar way as mesoderm.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Blotting, Northern
  • Cloning, Molecular
  • DNA / genetics
  • Embryonic Induction
  • Endoderm / physiology*
  • Gene Expression Regulation / drug effects
  • Genes, Homeobox*
  • Growth Substances / pharmacology
  • Mesoderm / physiology*
  • Molecular Sequence Data
  • Nucleic Acid Hybridization
  • RNA, Messenger / genetics
  • Time Factors
  • Tissue Distribution
  • Xenopus laevis / embryology*

Substances

  • Growth Substances
  • RNA, Messenger
  • DNA

Associated data

  • GENBANK/M27063