Modern hematology laboratories use automated hematology analyzers to perform cell counts. These instruments provide accurate, precise, low-cost differential counts with fast turnaround times. Technologies commonly used include electrical impedance, radiofrequency conductivity, laser light scattering, and cytochemistry. This article reviews the principles of these methodologies and possible sources of error, provides guidance for selecting flagging criteria, and discusses novel, clinically relevant white blood cell parameters provided by new instruments, including immature granulocyte count and granularity index.
Keywords: Automated hematology analyzer; Differential count; Laboratory instrumentation; Leukocyte; Methodology; White blood cell count.
Copyright © 2015 Elsevier Inc. All rights reserved.