Salidroside rescued mice from experimental sepsis through anti-inflammatory and anti-apoptosis effects

J Surg Res. 2015 May 1;195(1):277-83. doi: 10.1016/j.jss.2015.01.021. Epub 2015 Jan 14.

Abstract

Background: Salidroside (SDS) is the main effective component of Rhodiola rosea L with a variety of pharmacologic properties. The objective of this study was to investigate the efficacy of SDS in the treatment of experimental sepsis in mice and explore the possible underlying action mechanisms.

Methods: Sepsis was induced in C57BL/6 male mice via cecal ligation and puncture (CLP). The animals were divided into three groups as follows: sham, CLP, and CLP plus SDS. SDS (50 mg/kg) was injected intraperitoneally 1 h after operation. Postoperative survival of the mice, bacterial clearance in blood and peritoneal lavage fluid, cytokine secretion in blood, and histology of lung were evaluated. In addition, apoptosis of immune cells in the spleen and thymus were examined, respectively.

Results: SDS administration prolonged the survival of the septic mice, inhibited the proinflammatory responses, and enhanced bacterial clearance. It also alleviated the pathologic changes in the lung and inhibited the apoptosis of immune cells in the spleen and thymus after CLP challenge.

Conclusions: SDS exerts a protective effect in CLP-induced sepsis by attenuating the proinflammatory responses, enhancing bacterial clearance, and preserving adaptive immunity. SDS may be a promising therapeutic strategy for the treatment of sepsis.

Keywords: Apoptosis; Bacterial clearance; Experimental sepsis; Proinflammatory response; Traditional Chinese medicine.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acute Lung Injury / etiology
  • Acute Lung Injury / prevention & control
  • Animals
  • Apoptosis / drug effects
  • Cytokines / blood
  • Drug Evaluation, Preclinical
  • Glucosides / pharmacology
  • Glucosides / therapeutic use*
  • Inflammation / drug therapy
  • Male
  • Mice, Inbred C57BL
  • Phenols / pharmacology
  • Phenols / therapeutic use*
  • Phytotherapy*
  • Plant Extracts / pharmacology
  • Plant Extracts / therapeutic use
  • Random Allocation
  • Rhodiola*
  • Sepsis / blood
  • Sepsis / complications
  • Sepsis / drug therapy*
  • Spleen / drug effects
  • Thymus Gland / drug effects

Substances

  • Cytokines
  • Glucosides
  • Phenols
  • Plant Extracts
  • rhodioloside