Phlorofucofuroeckol A isolated from Ecklonia cava alleviates postprandial hyperglycemia in diabetic mice

Eur J Pharmacol. 2015 Apr 5:752:92-6. doi: 10.1016/j.ejphar.2015.02.003. Epub 2015 Feb 11.

Abstract

This study was designed to investigate whether phlorofucofuroeckol A inhibited α-glucosidase and α-amylase activities and alleviated postprandial hyperglycemia in diabetic mice. Phlorofucofuroeckol A that was isolated from Ecklonia cava (brown algae) demonstrated prominent inhibitory effects against α-glucosidase and α-amylase activities. The IC50 values of phlorofucofuroeckol A against α-glucosidase and α-amylase were 19.52 and 6.34μM, respectively. These inhibitory activities of phlorofucofuroeckol A were higher than those of acarbose, which was used as a positive control. Increases in postprandial blood glucose levels were significantly more suppressed in the group administered phlorofucofuroeckol A compared to the control group in both diabetic and normal mice. Moreover, the area under the curve was significantly lower after phlorofucofuroeckol A administration (2296 versus 2690mmolmin/l) in the diabetic mice. These results suggested that phlorofucofuroeckol A is a potent α-glucosidase inhibitor and can alleviate the postprandial hyperglycemia that is caused by starch.

Keywords: Diabetic mice; Phlorofucofuroeckol A; Postprandial hyperglycemia; α-glucosidase.

MeSH terms

  • Animals
  • Benzofurans / isolation & purification
  • Benzofurans / pharmacology*
  • Benzofurans / therapeutic use
  • Blood Glucose / metabolism
  • Diabetes Mellitus, Experimental / complications*
  • Dioxins / isolation & purification
  • Dioxins / pharmacology*
  • Dioxins / therapeutic use
  • Glycoside Hydrolase Inhibitors / isolation & purification
  • Glycoside Hydrolase Inhibitors / pharmacology*
  • Glycoside Hydrolase Inhibitors / therapeutic use
  • Hyperglycemia / blood
  • Hyperglycemia / complications*
  • Hyperglycemia / drug therapy*
  • Hyperglycemia / enzymology
  • Male
  • Mice
  • Mice, Inbred ICR
  • Phaeophyceae / chemistry*
  • alpha-Amylases / antagonists & inhibitors
  • alpha-Glucosidases / metabolism

Substances

  • Benzofurans
  • Blood Glucose
  • Dioxins
  • Glycoside Hydrolase Inhibitors
  • phlorofucofuroeckol A
  • alpha-Amylases
  • alpha-Glucosidases