High-performance liquid chromatography separation of the (S,S)- and (R,S)-forms of S-adenosyl-L-methionine

Jianyu Zhang; Judith P Klinman

doi:10.1016/j.ab.2015.02.004

High-performance liquid chromatography separation of the (S,S)- and (R,S)-forms of S-adenosyl-L-methionine

Anal Biochem. 2015 May 1:476:81-3. doi: 10.1016/j.ab.2015.02.004. Epub 2015 Feb 11.

Authors

Jianyu Zhang¹, Judith P Klinman²

Affiliations

¹ Department of Chemistry, University of California, Berkeley, Berkeley, CA 94720, USA; California Institute for Quantitative Biosciences, University of California, Berkeley, Berkeley, CA 94720, USA.
² Department of Chemistry, University of California, Berkeley, Berkeley, CA 94720, USA; California Institute for Quantitative Biosciences, University of California, Berkeley, Berkeley, CA 94720, USA; Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA. Electronic address: klinman@berkeley.edu.

Abstract

S-adenosyl-l-methionine (AdoMet), an important biological cofactor, exists in two chiral forms, (S,S)- and (R,S)-, only the former of which is biologically active. Here, we have developed a chromatographic method to obtain pure (S,S)-AdoMet using a single C18 column.

Keywords: Chiral resolution; Diastereoisomer; High-performance liquid chromatography; Radioactive; S-adenosyl-l-methionine; Separation.

Publication types

Research Support, N.I.H., Extramural

MeSH terms

Chromatography, High Pressure Liquid / methods*
S-Adenosylmethionine / isolation & purification*

Substances

S-Adenosylmethionine

Abstract

Publication types

MeSH terms

Substances

Grants and funding